Description

The monoclonal antibody specifically binds to the CD8 differentiation antigen of all mouse strains tested. A subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells) expresses almost exclusively the CD8 αβ heterodimer. Subsets of γδ TCR-bearing T cells, intestinal intrapithelial lymphocytes, and dendriticcells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to T lymphocyteswhich matured in the thymus or in an extrathymic environment that had been influenced by thymus-initiated neuroendocrine signals. CD8 isan antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells or epithelial cells.

Stability & Storage:

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μL experimental sample (a test).

2. Isotype control is not needed because the antibody has no Fc region.

For Research Use Only.

References

1. Schlossman SF, Boumsell L, Gilks W, et al, ed. Leukocyte Typing V: White Cell Differentiation Antigens. New York: Oxford University Press; 1995.

Knapp W, Dorken B, Rieber EP, et al, ed. Leucocyte Typing IV. New York: Oxford University Press; 1989.